Skip to main content

If a culture from a wound was sent to the lab for work up, explain the steps you would take to isolate and identify the causative agent. (Include...

Common Pathogens Found in Wounds


Staph. aureus, Beta hemolytic Strep. (S. pyogenes, S. agalactiae), E. coli, Proteus, Klebsiella, Pseudomonas, Acinetobacter, Stenotrophomonas, anaerobic bacteria


Wound Infection Progress and Gram Test Results


Particular types of bacteria may be suspected based on the age of the wound and the gram test results.



  • Early Acute Wound: Normal skin flora, followed by Staph. Aureus and Beta-hemolytic Strep.; mostly gram positive bacteria


  • About Four Weeks: facultative anaerobic gram negative...

Common Pathogens Found in Wounds


Staph. aureus, Beta hemolytic Strep. (S. pyogenes, S. agalactiae), E. coli, Proteus, Klebsiella, Pseudomonas, Acinetobacter, Stenotrophomonas, anaerobic bacteria


Wound Infection Progress and Gram Test Results


Particular types of bacteria may be suspected based on the age of the wound and the gram test results.



  • Early Acute Wound: Normal skin flora, followed by Staph. Aureus and Beta-hemolytic Strep.; mostly gram positive bacteria


  • About Four Weeks: facultative anaerobic gram negative rods such as Proteus, E. coli, and Klebsiella


  • Longer Term Wound: Anaerobic bacteria become more common


  • Long Term Chronic Wound: Often contain more anaerobic bacteria than aerobic bacteria - aerobic gram negative rods also seen; Examples include Pseudomonas, Acinetobacter, Stenotrophomonas

Common Culture Media


The following culture media can be used to isolate specific types of organisms:



  • Mannitol Salt Agar: Use to isolate Staphylococci and Micrococci


  • Phenylethyl Alcohol Agar: Use to isolate gram positive bacteria


  • Blood Agar: Use to grow most bacteria


  • Chocolate Agar: Used to isolate Neisseria and Haemophilus 

Common Enzymatic Tests



  • Catalase: Positive - Staphylococci; Negative - Streptococci and most anaerobes


  • Coagulase: Positive - Staphylococcus aureus


  • Oxidase: Positive - most gram negative bacteria

*Please also see the first reference listed below, which shows a detailed flowchart for the identification of bacteria based on differential culture media, enzymatic tests, and resistance patterns.



Comments

Post a Comment

Popular posts from this blog

What is the meaning of "juggling fiends" in Macbeth?

Macbeth is beginning to realize that the three witches have been deceiving him since he first encountered them. Like jugglers, they have kept changing their forecasts in order create confusion. This is particularly apparent when the Second Apparition they raise in Act IV,   Scene 1 tells him that no man of woman born can overcome him in hand-to-hand battle--and then Macbeth finds himself confronted by the one man he has been avoiding out of a... Macbeth is beginning to realize that the three witches have been deceiving him since he first encountered them. Like jugglers, they have kept changing their forecasts in order create confusion. This is particularly apparent when the Second Apparition they raise in Act IV,   Scene 1 tells him that no man of woman born can overcome him in hand-to-hand battle--and then Macbeth finds himself confronted by the one man he has been avoiding out of a sense of guilt, and that man tells him: Despair thy charm. And let the angel whom thou still hast serve...
Post a Comment
Read more

In Martel's Life of Pi, how does writing a diary help Pi Patel build on his leadership skills?

At the end of chapter 73, Pi first divulges the fact that he had started a diary after about a week alone at sea. Pi claims that he wrote about "practical stuff" (208) such as what he caught for food, foreseeable problems and solutions, Richard Parker, and reflections about specific events that occurred. In a sense, Pi is the captain of his ship; therefore, he must take responsibility for himself and his crew. He can... At the end of chapter 73, Pi first divulges the fact that he had started a diary after about a week alone at sea. Pi claims that he wrote about "practical stuff" (208) such as what he caught for food, foreseeable problems and solutions, Richard Parker, and reflections about specific events that occurred. In a sense, Pi is the captain of his ship; therefore, he must take responsibility for himself and his crew. He can do this by writing down events, problems and solutions, and reflections to look back on when confronted with repeated problems. It can ...
Post a Comment
Read more

Why did Tybalt attack and kill Mercutio instead of Romeo, who he had originally been targeting?

Though Tybalt's quarrel is with Romeo, he begins the momentous duel in Act 3 by fighting with and killing Mercutio instead. Tybalt ultimately attacks Mercutio because the garrulous and hot-headed character insults Tybalt and goads him into a duel to protect Romeo's honor. From the beginning of the encounter in Act 3, Scene 1, it's clear that Mercutio is itching for a fight. For instance, when Tybalt signals that he wants to talk to Mercutio... Though Tybalt's quarrel is with Romeo, he begins the momentous duel in Act 3 by fighting with and killing Mercutio instead. Tybalt ultimately attacks Mercutio because the garrulous and hot-headed character insults Tybalt and goads him into a duel to protect Romeo's honor. From the beginning of the encounter in Act 3, Scene 1, it's clear that Mercutio is itching for a fight. For instance, when Tybalt signals that he wants to talk to Mercutio and Benvolio, Mercutio responds "And but one word with one of us?/ Couple it w...
Post a Comment
Read more